Cuticular hydrocarbon analysis in forensic entomology
A Review
DOI:
https://doi.org/10.1558/aefs.36241Keywords:
Forensic Entomology, PMI estimations, Blowflies, Cuticular Hydrocarbon AnalysisAbstract
Blowflies are the first inhabitants of decomposing remains and are therefore of forensic relevance for post mortem interval estimations. Forensic entomology is becoming widely accepted as a branch of forensic science and is being utilised more within forensic casework. This wider use has driven an increase in research being carried out within the field, in particular, in less “classical” techniques such as DNA and chemical analysis in the form of cuticular hydrocarbon analysis. This short review will examine the research currently being studied in the area of cuticular hydrocarbon analysis of forensically important Diptera for species identification and ageing.
References
Adams, Z. & Hall, M.J.R. (2003) Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length. Forensic Science International, 138, 50–61.
Akino, T., Yamamura, K., Wakamura, S. & Yamaoka, R. (2004) Direct behavioral evidence for hydrocarbons as nestmate recognition cues in Formica japonica (Hymenoptera: Formicidae). Applied Entomology and Zoology, 39, 381–387.
Amendt, J., Krettek, R., Niess, C., Zehner, R. & Bratzke, H. (2000) Forensic entomology in Germany. Forensic Science International, 113, 309–14.
Ames, C., Turner, B. & Daniel, B. (2006a) Estimating the post-mortem interval (I): The use of genetic markers to aid in identification of Dipteran species and subpopulations. International Congress Series, 1288, 795–797.
Ames, C., Turner, B. & Daniel, B. (2006b) The use of mitochondrial cytochrome oxidase I gene (COI) to differentiate two UK blowfly species - Calliphora vicina and Calliphora vomitoria. Forensic science international, 164, 179–82.
Benecke, M. (2001) A brief history of forensic entomology. Forensic Science International, 120, 2–14.
Bernier, U.R., Carlson, D.A. & Geden, C.J. (1998) Analysis of the Cuticular Hydrocarbons from Parasitic Wasps of the Genus Muscidifurax. Journal of the American Society for Mass Spectrometry, 9, 320–332.
Blomquist, G.J. & Bagnères, A.G. (Eds.). (2010) Insect Hydrocarbons: Biology, Biochemistry, and Chemical Ecology. Cambridge University Press.
Braga, M.V., Bragaa, Pintob, Z.T., de Carvalho Queiroza, M.M., Matsumotod, N. & Blomquist, G.J. (2013) Cuticular hydrocarbons as a tool for the identification of insect species: Puparial cases from Sarcophagidae. Acta Tropica, 128, 479– 485.
Braga, M.V., Bragaa, Pintob, Z.T., de Carvalho Queiroza, M.M., Matsumotod, N. & Blomquist, G.J. (2016) Effect of age on cuticular hydrocarbon profiles in adult Chrysomya putoria (Diptera: Calliphoridae). Forensic Science Internaitonal, 259, e37-e47
Brown, W.V., Morton, R. & Spradbery, J.P. (1992) Cuticular hydrocarbons of the Old World screw-worm fly, Chrysomya bezziana Villeneuve (Diptera: Calliphoridae). Chemical characterization and quantification by age and sex. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 101, 665–671.
Brown, W.V., Rose, H.A., Lacey, M.J. & Wright, K. (2000) The cuticular hydrocarbons of the giant soil-burrowing cockroach Macropanesthia rhinoceros saussure (Blattodea: Blaberidae: Geoscapheinae): analysis with respect to age, sex and location. Comparative Biochemistry and Physiology. Part B, Biochemistry & molecular biology, 127, 261–77.
Butcher, J.B., Moore, H.E., Day, C.R., Adam, C.D. & Drijfhout, F.P. (2013) Artificial Meural Network analysis of hydrocarbon profiles for the ageing of Lucilia sericata for Post Mortem Interval estimation. Forensic Science International, 232, 25-31.
Cainé, L.M., Real, F.C., Saloña-Bordas, M.I., Pancorbo, M.M. de, Lima, G., Magalhães, T. & Pinheiro, F. (2009) DNA typing of Diptera collected from human corpses in Portugal. Forensic Science International, 184, e21–23.
Chapman, R.F. & Espelies, K.E. (1995) Use of Cuticular Lipids in Grasshopper Taxonomy: A Study of Variation in Schistocerca shoshone (Thomas). Biochemical Systematics and Ecology, 23, 383–398.
Chen, C.S., Mulla, M.S., March, R.B. & Chaney, .J.D. (1990) Cuticular hydrocarbon Patterns in Culex-quinquefasciatus as influenced by age and sex and geography. Bulletin of the Society for Vector Ecology, 15, 129–139.
Desena, M.L., Clark, J.M., Edman, J.D., Symington, S.B., Scott, T.W., Clark, G.G. & Peters, T.M. (1999) Potential for ageing Female Aedes aegypti (Diptera: Culicidae) by Gas Chromatographic Analysis of Cuticular Hydrocarbons, Including a Field Evaluaiton. Journal of Medical Entomology, 36, 811–823.
Desena, M.L., Edman, J.D., Clark, J.M., Symington, S.B. & Scott, T.W. (1999) Aedes aegypti (Diptera: Culicidae) Age Determination by Cuticular Hydrocarbon Analysis of Female Legs. Journal of Medical Entomology, 36, 824–830.
Everaerts, C., Farine, J.P. & Brossut, R. (1997) Changes of species specific cuticular hydrocarbon profiles in the cockroaches Nauphoeta cinerea and Leucophaea maderae reared in heterospecific groups. Behavioral Ecology and Sociobiology, 85, 145–150.
Gennard, D.E. (2007) Forensic Entomology. John Wiley & Sons Ltd. Gibbs, A.G. (1998) Water-Proofing Properties of Cuticular Lipids. American Zoologist, 38, 471–482.
Gibbs, A.G. & Crockett, E.L. (1998) The Biology of Lipids: Integrative and Comparative Perspectives. Integrative and Comparative Biology, 38, 265–267.
Guillem, R.M., Drijfhout, F.P. & Martin, S.J. (2012) Using chemo-taxonomy of host ants to help conserve the large blue butterfly. Biological Conservation, 148, 39–43.
Hart, A.J., Whitaker, A.P. & Hall, M.J.R. (2008) The Use of Forensic Entomology in Criminal Investigations: How it can be of benefit to SIOs. The Journal of Homicide and Major Incident Investigation, 4, 37–48.
Harvey, M.L., Dadour, I.R. & Gaudieri, S. (2003) Mitochondrial DNA cytochrome oxidase I gene: potential for distinction between immature stages of some forensically important fly species (Diptera) in western Australia. Forensic Science International, 131, 134–9.
Haverty, M.I., Collins, M.S., Nelson, L.J. & Thorne, B.L. (1997) Cuticular Hydrocarbons of Termites of the British Virgin Islands. Journal of Chemical Ecology, 23, 927–964.
Howard, R.W. & Blomquist, G.J. (2005) Ecological, behavioral, and biochemical aspects of insect hydrocarbons. Annual review of entomology, 50, 371–93.
Hugo, L.E., Kay, B.H., Eaglesham, G.K., Holling, N. & Ryan, P.A. (2006) Investigation of cuticular hydrocarbons for determining the age and survivorship of australasian mosquitoes. The American Journal of Tropical Medicine and Hygiene, 74, 462–74.
Jackson, L.L. & Bartelt, R.J. (1986) Cuticular Hydrocarbons of Sex. Insect Biochemistry, 16, 433–439.
Lavine, B.K. & Vora, M.N. (2005) Identification of Africanized honeybees. Journal of Chromatography. A, 1096, 69–75.
Lockey, K.H. (1992) Insect hydrocarbon chemotaxonomy: Cuticular hydrocarbons of adult and larval epiphysa species blanchard and adult Onymacris unguicularis (HAAG) (tenebrionidae: Coleoptera). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 102, 451–470.
Lockey, K.H. (1991) Insect hydrocarbon classes: Implications for chemotaxonomy. Insect Biochemistry, 21, 91–97.
Malgorn, Y. & Coquoz, R. (1999) DNA typing for identification of some species of Calliphoridae. An interest in forensic entomology. Forensic Science International, 102, 111–9.
Martin, S.J. & Drijfhout, F.P. (2009) How reliable is the analysis of complex cuticular hydrocarbon profiles by multivariate statistical methods? Journal of Chemical Ecology, 35, 375–82.
Martin, S.J., Helanterä, H. & Drijfhout, F.P. (2008) Colony-specific hydrocarbons identify nest mates in two species of Formica ant. Journal of Chemical Ecology, 34, 1072–80.
Martin, S., Vitikainen, E., Helanterä, H. & Drijfhout, F. (2008) Chemical basis of nest-mate discrimination in the ant Formica exsecta. Proceedings of the Royal Soceity B: Biological Sciences, 275, 1271–1278.
Moore, H.E. (2013) Analysis of cuticular hydrocarbons in forensically important blowflies using mass spectrometry and its application in Post Mortem Interval estimations. PhD thesis, Keele University, UK.
Moore, H.E., Adam, C.D. & Drijfhout, F.P. (2013) Potential Use of Hydrocarbons for Aging Lucilia sericata Blowfly Larvae to Establish the Postmortem Interval. Journal of Forensic Sciences, 58, 404-412.
Moore, H.E., Adam, C.D. & Drijfhout, F.P. (2014) Identifying 1st instar larvae for three forensically important blowfly species using "fingerprint" cuticular hydrocarbon analysis. Forensic Science International, 240, 48-53.
Moore, H.E., Butcher, J.B., Adam, C.D., Day, C.R., Drijfhout, F.P. (2016) Age estimation of Calliphora (Diptera: Calliphoridae) larvae using cuticular hydrocarbon analysis and Artificial Neural Networks. Forensic Science International, 268, 81-91.
Moore, H.E., Butcher, J.B., Adam, C.D., Day, C.R., Drijfhout, F.P. (2016) Age estimation of Calliphora (Diptera: Calliphoridae) larvae using cuticular hydrocarbon analysis and Artificial Neural Networks. Forensic Science International, 268, 81-91.
Moore, H.E., Butcher, J.B., Day, C.R., Drijfhout, F. P. (2017) Adult fly age estimations using cuticular hydrocarbons and Artificial Neural Networks in forensically important Calliphoridae species. Forensic Science International, 280, 233-244.
Moore, H.E., Pechal, J.L., Benbow, M.E. Drijfhout, F.P. (2017) The potential use of cuticular hydrocarbons and multivariate analysis to age empty puparial cases of Calliphora vicina and Lucilia sericata. Scientific Reports, 7:1933.
Mpuru, S., Blomquist, G.J., Schal, C., Roux, M., Kuenzli, M., Dusticier, G., Clément, J.L. & Bagnères, A.G. (2001) Effect of age and sex on the production of internal and external hydrocarbons and pheromones in the housefly, Musca domestica. Insect Biochemistry and Molecular Biology, 31, 139–55.
Musah, R.A., Espinoza, E.O., Cody, R.B., Lesiak, A.D., Christensen, E.D., Moore, H.E., Maleknia, S. & Drijfhout, F.P. (2015) A high throughput ambient mass spectrometric approach to species identification and classification from chemical fingerprint signatures. Scientific Reports, 5, 1-16.
Page, M., Nelson, L.J., Blomquist, G.J. & Seybold, S.J. (1997) Cuticular Hydrocarbons as Chemotaxonomic Characters of Pine Engraver Beetles (Ips spp.) in the grandicollis Subgeneric Group. Journal of Chemical Ecology, 23, 1053–1099.
Pechal, J.L., Moore, H.E., Drijfhout, F.P., Benbow, M.E. (2014) Hydrocarbon profiles throughout adult Calliphoridae aging: A promising tool for forensic entomology. Forensic Science International, 245, 65-71.
Ratcliffe, S.T., Webb, D.W., Weinzievr, R.A. & Robertson, H.M. (2003) PCR-RFLP identification of Diptera (Calliphoridae, Muscidae and Sarcophagidae)--a generally applicable method. Journal of Forensic Sciences, 48, 783–5.
Roux, O., Gers, C. & Legal, L. (2008) Ontogenetic study of three Calliphoridae of forensic importance through cuticular hydrocarbon analysis. Medical and Veterinary Entomology, 22, 309–17.
Sperling, F.A., Anderson, G.S. & Hickey, D.A. (1994) A DNA-based approach to the identification of insect species used for postmortem interval estimation. Journal of Forensic Sciences, 39, 418–27.
Steiner, S., Hermann, N. & Ruther, J. (2006) Characterization of a female-produced courtship pheromone in the parasitoid Nasonia vitripennis. Journal of Chemical Ecology, 32, 1687–702.
Stevens, J. & Wall, R. (2001) Genetic relationships between blowflies (Calliphoridae) of forensic importance. Forensic Science International, 120, 116–123.
Szpila, K. (2010) Key for the Identification of Third Instars of European Blowflies (Diptera: Calliphoridae) of Forensic Importance. Current Concepts in Forensic Entomology. (eds J. Amendt, C.P. Campobasso, M.L. Goff & M. Grassberger), p. 47. Springer.
Szpila, K., Hall, M.J.R., Pape, T. & Grzywacz, a. (2012) Morphology and identification of first instars of the European and Mediterranean blowflies of forensic importance. Part II. Luciliinae. Medical and veterinary entomology, 87–100.
Tissot, M., Nelson, D.R. & Gordon, D.M. (2001) Qualitative and quantitative differences in cuticular hydrocarbons between laboratory and field colonies of Pogonomyrmex barbatus. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 130, 349–58.
Trabalon, M., Campan, M., Clement, J.L., Lange, C. & Miquel, M.T. (1992) Cuticular Hydrocarbons of Calliphora vomitoria (Diptera): Relation to Age and Sex. General and Comparative Endocrinology, 85, 208–216.
Tregenza, T., Buckley, S.H., Pritchard, V.L. & Butlin, R.K. (2000) Inter- and Intra-Population Effects of Sex and Age on Epicticular Composition of Meadow Grasshopper,Chorthippus parallelus. Journal of Chemical Ecology, 26, 257–278.
Urech, R., Brown, G.W., Moore, C.J. & Green, P.E. (2005) Cuticular hydrocarbons of buffalo fly, Haematobia exigua, and chemotaxonomic differentiation from horn fly, H. irritans. Journal of Chemical Ecology, 31, 2451–61.
Vincent, S., Vian, J.M. & Carlotti, M.P. (2000) Partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem interval estimation. Journal of Forensic Sciences, 45, 820–3.
Wallman, J.F. & Donnellan, S.C. (2001) The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera : Calliphoridae) in southeastern Australia. Forensic Science International, 120, 60–67.
Wells, J.D. & Sperling, F.A.H. (2001) DNA-based identification of forensically important Chrysomyinae (Diptera : Calliphoridae). Forensic Science International, 120, 110–115.
Ye, G., Li, K., Zhu, J., Zhu, G. & Hu, C. (2007) Cuticular hydrocarbon composition in pupal exuviae for taxonomic differentiation of six necrophagous flies. Journal of Medical Entomology, 44, 450–6.
Yew, J.Y., Cody, R.B. & Kravitz, E. a. (2008) Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America, 105, 7135–40.
Zhu, G.H., Xu, X.H., Yu, X.J., Zhang, Y. & Wang, J.F. (2007) Puparial case hydrocarbons of Chrysomya megacephala as an indicator of the postmortem interval. Forensic Science International, 169, 1–5.
Zhu, G.H., Ye, G.Y., Hu, C., Xu, X.H. & Li, K. (2006) Development changes of cuticular hydrocarbons in Chrysomya rufifacies larvae: potential for determining larval age. Medical and Veterinary Entomology, 20, 438–44.
Zhu, G.H., Yu, X.J., Xie, L.X., Luo, H.,Wang, D., Lv, J.Y. & Xu, X.H (2013) Time of Death Revealed by Hydrocarbons of Empty Puparia of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae): A Field Experiment. PLoS ONE, 8, 1-7.
Zhu, G.H., Jia, Z.J., Yu, X.J., Wu, K.S., Chen, L.S., Lv, J.Y. & Benbow, M.E. (2017) Predictable weathering of puparial hydrocarbons of necrophagous flies for determining the postmortem interval: a field experiment using Chrysomya rufifacies. International Journal of Legal Medicine, 131, 885–894.
