The Value of an Empirical Approach for the Assessment of Diatoms as Environmental Trace Evidence in Forensic Limnology
DOI:
https://doi.org/10.1558/aefs.32474Keywords:
environmental trace evidence; freshwater algae; diatom analysis; crime scene investigation; empirical evidence base; Scanning Electron Microscopy, environmental trace evidence, freshwater algae, diatom analysis, crime scene investigation, empirical evidence base, scanning elecron microscopyAbstract
Environmental trace evidence is often encountered during a forensic investigation and is acknowledged to have the potential to contribute valuable circumstantial information pertaining to the context of an individual criminal event. Although traditional study has focused upon the analysis of terrestrial soil and sediment traces, there is growing potential for the forensic assessment of aquatic crime scenes, particularly those within freshwater environments. This paper outlines the current applications of limnology, particularly algae and diatom analysis, within forensic science and introduces new and ongoing research within the field. Two empirical studies are presented which highlight the importance of developing evidence bases within freshwater trace evidence analysis. These studies demonstrate the analytical capability of the Scanning Electron Microscope (SEM) at various stages of an investigation: in the initial screening and collection of an evidential sample from clothing (1); and in the analysis of preserved diatoms following various levels of their exposure to fire damage (2). The results highlight that the SEM provides a valuable tool during the initial stages of an investigation, determining the presence and abundance of a range of environmental indicators and directing further strategy for the more in-depth collection and analysis of a forensic sample. Furthermore, the preservation of diatoms adhering to clothing following prolonged exposure to fire, indicates that efforts to collect any destroyed evidence are worthwhile given the potential to recover freshwater traces over extended time scales. Finally, the value of adopting an empirical approach for the development of a forensically relevant evidence base within forensic limnology, and the importance of having an appreciation of the legal implications for the interpretation and admissibility of freshwater evidence is presented.
References
Anderson, G. S. and L. S. Bell. 2016. “Impact of marine submergence and season on faunal colonization and decomposition of pig carcasses in the Salish Sea.” PloS One 11(3): e0149107. https://doi.org/10.1371/journal.pone.0149107
Annual Report of the Government Chief Scientific Adviser. 2015. Forensic Science and Beyond: Authenticity, Provenance and Assurance. The Government Office for Science, London
Auer, A. and M. Möttönen. 1988. “Diatoms and drowning.” Zeitschrift für Rechtsmedizin 101 (2): 87–98. https://doi.org/10.1007/bf00200290
Battarbee, R. W., V. J. Jones, R. J. Flower, N. G. Cameron, H. Bennion, L. Carvalho and S. Juggins. 2001. “Diatoms.” In Tracking Environmental Change Using Lake Sediments Volume 3: Terrestrial, Algal, and Siliceous Indicators, edited by J. P. Smol, H. J. B. Birks and W. M. Last, 155–202. Dordrecht: Kluwer Academic Publishers.
Birks, H. J. B., V. J. Jones and N. L. Rose. 2004. “Recent environmental change and atmospheric contamination on Svalbard as recorded in lake sediments–synthesis and general conclusions.” Journal of Paleolimnology 31(4): 531–546. https://doi.org/10.1023/B:JOPL.0000022550.81129.1a
Black, B., F. J. Ayala and C. Saffran-Brinks. 1993. “Science and the law in the wake of Daubert: A new search for scientific knowledge.” Texas Law Review 72: 715.
Brown, A. G. 2006. “The use of forensic botany and geology in war crimes investigations in NE Bosnia.” Forensic Science International 163(3): 204–210. https://doi.org/10.1016/j.forsciint.2006.05.025
Bull, P. A., A. Parker and R. M. Morgan. 2006. “The forensic analysis of soils and sediment taken from the cast of a footprint.” Forensic Science International 162(1): 6–12. https://doi.org/10.1016/j.forsciint.2006.06.075
Cameron, N. G. 2004. “The use of diatom analysis in forensic geoscience.” In Forensic Geoscience: Principles, Techniques and Applications, edited by K. Pye and D. J. Croft, 277–280. Geological Society London, Special Publications 232. London: Geological Society. https://doi.org/10.1144/gsl.sp.2004.232.01.25
Carlie, A., C. Arcini, H. Druid and J. Risberg. 2014. “Archaeology, forensics, and the death of a child in Late Neolithic Sweden.” Antiquity 88: 1148–1163. https://doi.org/10.1017/S0003598X00115376
Casamatta, D. A. and R. G. Verb. 2000. “Algal colonization of submerged carcasses in a mid-order woodland stream.” Journal of Forensic Science 45(6): 1280–1285. https://doi.org/10.1520/jfs14880j
Codd, G. A. 2000. “Cyanobacterial toxins, the perception of water quality, and the prioritisation of eutrophication control.” Ecological Engineering 16(1): 51–60. https://doi.org/10.1016/S0925-8574(00)00089-6
Cox. E. J. 2012. “Diatoms in forensic science.” In Forensic Ecology Handbook: From Crime Scene to Court, edited by N. Marquez-Grant and J. Roberts, 141–151. Oxford: Wiley. https://doi.org/10.1002/9781118374016.ch9
Dawson, L. A. and S. Hillier. 2010. “Measurement of soil characteristics for forensic applications.” Surface and Interface Analysis 42(5): 363–377. https://doi.org/10.1002/sia.3315
Delabarde, T., C. Keyser, A. Tracqui, D. Charabidze and B. Ludes. 2013. “The potential of forensic analysis on human bones found in riverine environment.” Forensic Science International 228(1): e1–e5. https://doi.org/10.1016/j.forsciint.2013.03.019
Gessner, B. D., J. P. Middaugh and G. J. Doucette. 1997. “Paralytic shellfish poisoning in Kodiak, Alaska.” Western Journal of Medicine 166: 351–353.
Graham, L. E. and L. W. Wilcox. 2000. Algae. Upper Saddle River, NJ: Prentice Hall.
Graham, J. L., K. A. Loftin, M. T. Meyer and A. C. Ziegler. 2010. “Cyanotoxin mixtures and taste-andodor compounds in cyanobacterial blooms from the midwestern United States.” Environmental Science Technology 44: 7361–7368. https://doi.org/10.1021/es1008938
Haefner, J., J. R. Wallace and R. W. Merritt. 2004. “Pig decomposition in lotic aquatic systems: The potential use of algal growth in establishing a postmortem submersion interval (PMSI).” Journal of Forensic Science 49(2): 330–336. https://doi.org/10.1520/jfs2003283
Hardy, C. R. and J. R. Wallace. 2012. “Algae in forensic investigations.” In Forensic Botany: A Practical Guide, edited by D. W. Hall and J. H. Byrd, 145–173. Oxford: Wiley-Blackman. https://doi.org/10.1002/9781119945734.ch9
Hawksworth, D. L. and P. E. Wiltshire. 2011. “Forensic mycology: The use of fungi in criminal investigations.” Forensic Science International 206(1): 1–11. https://doi.org/10.1016/j.forsciint.2010.06.012
Hoek, C., D. Mann and H. M. Jahns. 1995. Algae: An introduction to phycology. Cambridge: Cambridge University Press.
Holstege, C. P. 2011. “Saxatoxin.” In Criminal Poisoning: Clinical and Forensic Perspectives, edited by C. P. Holstege, T. M. Neer, G. B. Saathoff and R. B. Furbee, 141–144. Sudbury, MA: Jones and Bartlett.
Hürlimann, J., P. Feer, F. Elber, K. Niederberger, R. Dirnhofer and D. Wyler. 2000. “Diatom detection in the diagnosis of death by drowning.” International Journal of Legal Medicine 114(1–2): 6–14. https://doi.org/10.1007/s004149900122
Incze, G. 1942. “Fremdkurper in Blutkreislauf Ertrunkener.” Zentralblatt für allgemeine Pathologie und pathologische Anatomie 79: 176.
Inman, K. and N. Rudin. 2002. “The origin of evidence.” Forensic Science International 126(1): 11–16. https://doi.org/10.1016/S0379-0738(02)00031-2
Jansma, M. J. 1981. “Diatom analysis from coastal sites in the Netherlands.” In Environmental Aspects of Coasts and Islands, edited by D. Brothwell and G. Dimbleby, 145–162. British Archaeological Reports International series 94. Oxford: British Archaeological Reports.
Johansen, J. R. 2010. “Diatoms of aerial habitats.” In The Diatoms: Applications for the Environmental and Earth Sciences, edited by E. P. Stoermer and J. P. Smol, 465–472. Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511763175.025
Jones, V. J. 2007. “Diatom Introduction.” In Encyclopedia of Quaternary Science, edited by S. Elias, 476–484. Oxford: Elsevier. https://doi.org/10.1016/B0-44-452747-8/00232-5
Juggins, S. and H. J. B. Birks. 2012. “Quantitative environmental reconstructions from biological data.” In Tracking Environmental Change Using Lake Sediments Volume 3: Terrestrial, Algal, and Siliceous Indicators, edited by J. P. Smol, H. J. B. Birks and W. M. Last, 431–494. Dordrecht: Kluwer Academic Publishers. https://doi.org/10.1007/978-94-007-2745-8_14
Kakizaki, E. and N. Yukawa. 2015. “Simple protocol for extracting diatoms from lung tissues of suspected drowning cases within 3h: First practical application.” Forensic Science International 251: 179–185. https://doi.org/10.1016/j.forsciint.2015.03.025
Keiper, J. B. and D. A. Casamatta. 2001. “Benthic organisms as forensic indicators.” Journal of the North American Benthological Society 20(2): 311–324. https://doi.org/10.2307/1468325
Krstic, S., A. Duma, B. Janevska, Z. Levkov, K. Nikolova and M. Noveska. 2002. “Diatoms in forensic expertise of drowning—a Macedonian experience.” Forensic Science International 127(3): 198–203. https://doi.org/10.1016/S0379-0738(02)00125-1
Law Commission. 2011. Expert evidence in criminal proceedings in England and Wales (Vol. 829). London: The Stationery Office.
Magni, P. A., C. Venn, I. Aquila, F. Pepe, P. Ricci, C. Di Nunzio and I. R. Dadour. 2015. “Evaluation of the floating time of a corpse found in a marine environment using the barnacle Lepas anatifera L.(Crustacea: Cirripedia: Pedunculata).” Forensic Science International 247: e6–e10. https://doi.org/10.1016/j.forsciint.2014.11.016
Merritt, R. W. and J. R. Wallace. 2000. “The role of aquatic insects in forensic investigations.” In Forensic Entomology: The utility of arthropods in legal investigations, edited by J. H. Byrd and J. L. Castner, 177–222. Boca Raton, FL: CRC Press. https://doi.org/10.1201/9781420036947.ch6
Mildenhall, D. C. 2006. “Hypericum pollen determines the presence of burglars at the scene of a crime: An example of forensic palynology.” Forensic Science International 163(3): 231–235. https://doi.org/10.1016/j.forsciint.2005.11.028
Ming, M., X. Meng and E. Wang. 2007. “Evaluation of four digestive methods for extracting diatoms.” Forensic Science International 170(1): 29–34. https://doi.org/10.1016/j.forsciint.2006.08.022
Mnookin, J., S. A. Cole, I. Dror, B. A. Fisher, M. Houk, K. Inman and N. Rudin. 2011. “The need for a research culture in the forensic sciences.” UCLA Law Review 58(3): 725–780.
Morgan, R. M., P. Wiltshire, A. Parker and P. A. Bull. 2006. “The role of forensic geoscience in wildlife crime detection.” Forensic Science International 162(1): 152–162. https://doi.org/10.1016/j.forsciint.2006.06.045
Morgan, R. M. and P. A. Bull. 2007a. “Forensic geoscience and crime detection.” Minerva Medicolegale 127: 73–89.
———. 2007b. “The philosophy, nature and practice of forensic sediment analysis.” Progress in Physical Geography 31(1): 43–58. https://doi.org/10.1177/0309133307073881
———. 2014. “Forensic environmental evidence.” In Encyclopedia of Criminology and Criminal Justice, edited by G. Bruinsma and D. Weisburd, 1705–1713. New York: Springer. https://doi.org/10.1007/978-1-4614-5690-2_168
Morgan, R. M., M. Little, A. Gibson, L. Hicks, S. Dunkerley and P. A. Bull. 2008. “The preservation of quartz grain surface textures following vehicle fire and their use in forensic enquiry.” Science and Justice 48(3): 133–140. https://doi.org/10.1016/j.scijus.2008.04.001
Morgan, R. M., J. Cohen, I. McGookin, J. Murly-Gotto, R. O’Connor, S.Muress and P. A. Bull. 2009. “The relevance of the evolution of experimental studies for the interpretation and evaluation of some trace physical evidence.” Science and Justice 49(4): 277–285. https://doi.org/10.1016/j.scijus.2009.02.004
Morgan, R. M., J. Flynn, V. Sena and P. A. Bull. 2014a. “Experimental forensic studies of the preservation of pollen in vehicle fires.” Science and Justice 54(2): 141–145. https://doi.org/10.1016/j.scijus.2013.04.001
Morgan, R. M., E. Allen, T. King and P. A. Bull. 2014b. “The spatial and temporal distribution of pollen in a room: Forensic implications.” Science and Justice 54(1): 49–56. https://doi.org/10.1016/j.scijus.2013.03.005
National Research Council. 2009. “Strengthening forensic science in the United States: A path forward.” Hearing before the Subcommittee on Crime, Terrorism, and Homeland Security of the Committee on the Judiciary, House of Representatives, One Hundred Eleventh Congress, first session, May 13.
Newell, A. J., R. M. Morgan, L. D. Griffin, P. A. Bull, J. R. Marshall and G. Graham. 2012. “Automated texture recognition of quartz sand grains for forensic applications.” Journal of Forensic Sciences 57(5): 1285–1289. https://doi.org/10.1111/j.1556-4029.2012.02126.x
Pagliaro, E. 2005. “Additional case studies.” In Forensic Botany: Principles and Applications in Criminal Casework, edited by H. M. Coyle, 179–183. Boca Raton, FL: CRC Press.
Peabody, A. J. 1971. “A case of safebreaking involving diatomaceous safe-ballast.” Journal of the Forensic Science Society 11(4): 227. https://doi.org/10.1016/S0015-7368(71)70665-3
———. 1977. “Diatoms in forensic science.” Journal of the Forensic Science Society 17(2): 81–87. https://doi.org/10.1016/S0015-7368(77)71130-2
———. 1980. “Diatoms and drowning—a review.” Medicine, Science and the Law 20(4): 254–261.
Peabody, A. J. and N. G. Cameron. 2010. “Forensic science and diatoms”. In The Diatoms: Applications for the Environmental and Earth Sciences, edited by E. P. Stoermer and J. P. Smol, 534–540. Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511763175.030
Piperno, D. R. 2006. Phytoliths: A Comprehensive Guide for Archaeologists and Paleoecologists. Lanham, MD: Altamira.
Pirrie, D., M. R. Power, G. K. Rollinson, P. E. Wiltshire, J. Newberry and H. E. Campbell. 2009. “Automated SEM-EDS (QEMSCAN®) Mineral analysis in forensic soil investigations: Testing instrumental reproducibility.” In Criminal and Environmental Soil Forensics, edited by K. Ritz, L. Dawson and D. Miller, 411–430. Dordrecht: Springer. https://doi.org/10.1007/978-1-4020-9204-6_26
Pollanen, M. S. 1997. “The diagnostic value of the diatom test for drowning, II. Validity: analysis of diatoms in bone marrow and drowning medium.” Journal of Forensic Science 42(2): 286–290. https://doi.org/10.1520/jfs14112j
———. 1998. Forensic Diatomology and Drowning. Amsterdam: Elsevier.
Pringle, J. K., A. Ruffell, J. R. Jervis, L. Donnelly, J. McKinley, J. Hansen and M. Harrison. 2012. “The use of geoscience methods for terrestrial forensic searches.” Earth Science Reviews 114(1): 108–123. https://doi.org/10.1016/j.earscirev.2012.05.006
Renberg, I. 1990. “A procedure for preparing large sets of diatom slides from sediment cores.” Journal of Paleolimnology 4(1): 87–90. https://doi.org/10.1007/bf00208301
Reynolds, C. S. 2006. The Ecology of Phytoplankton. Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511542145
Riding, J. B., B. G. Rawlins and K. H. Coley. 2007. “Changes in soil pollen assemblages on footwear worn at different sites.” Palynology 31(1): 135–151. https://doi.org/10.2113/gspalynol.31.1.135
Round, F. E. 1970. Biology of the Algae. London: Arnold.
Round, F. E., R. M. Crawford and D. G. Mann. 1990. Diatoms: Biology and Morphology of the Genera. Cambridge: Cambridge University Press.
Ruffell, A. 2010. “Forensic pedology, forensic geology, forensic geoscience, geoforensics and soil forensics.” Forensic Science International 202(1): 9–12. https://doi.org/10.1016/j.forsciint.2010.03.044
Ruffell, A. and J. McKinley. 2005. “Forensic geoscience: Applications of geology, geomorphology and geophysics to criminal investigations.” Earth Science Reviews 69(3): 235–247. https://doi.org/10.1016/j.earscirev.2004.08.002
Scott, K. R., R. M. Morgan, V. J. Jones and N. G. Cameron. 2014. “The transferability of diatoms to clothing and the methods appropriate for their collection and analysis in forensic geoscience.” Forensic Science International 241: 127–137.
Siver, P. A., W. D. Lord and D. J. McCarthy. 1994. “Forensic limnology: The Use of freshwater algal community ecology to link suspects to an aquatic crime scene in southern New England.” Journal of Forensic Sciences 39(3): 847–853. https://doi.org/10.1520/jfs13663j
Smith, A., A. G. Brown and O. Elmhurst. 2002. “The combined use of pollen and soil analyses in a search and subsequent murder investigation.” Journal of Forensic Science 47(3): 614–618. https://doi.org/10.1016/j.forsciint.2014.05.011
Stam, M. 2009. “The value of diatoms and botanical evidence in a police brutality case.” Paper presented at the National Institute of Justice Trace Evidence Symposium, Clearwater Beach, Florida, August 7-9, 2009.
Stoney, D. A. and P. L. Stoney. 2015. “Critical review of forensic trace evidence analysis and the need for a new approach.” Forensic Science International 251: 159–170. https://doi.org/10.1016/j.forsciint.2015.03.022
Uitdehaag, S., A. Dragutinovic and I. Kuiper. 2010. “Extraction of diatoms from (cotton) clothing for forensic comparisons.” Forensic Science International 200(1): 112–116. https://doi.org/10.1016/j.forsciint.2010.03.039
US Geological Survey (USGS). “How much water is there on, in, and above the Earth?” USGS.gov. http://water.usgs.gov/edu/earthhowmuch.html. Accessed May 3, 2016.
Wiltshire, P. E. 2006. “Hair as a source of forensic evidence in murder investigations.” Forensic Science International 163(3): 241–248. https://doi.org/10.1016/j.forsciint.2006.06.070
———. 2009. “Forensic ecology, botany, and palynology: some aspects of their role in criminal investigation.” In Criminal and Environmental Soil Forensics, edited by K. Ritz, L. Dawson and D. Miller, 129–149. Dordrecht: Springer. https://doi.org/10.1007/978-1-4020-9204-6_9
———. 2015. “Protocols for forensic palynology.” Palynology 40(1): 4–24. https://doi.org/10.1080/01916122.2015.1091138
Yen, L. Y., and P. T. Jayaprakash. 2007. “Prevalence of diatom frustules in non-vegetarian foodstuffs and its implications in interpreting identification of diatom frustules in drowning cases.” Forensic Science International 170(1): 1–7. https://doi.org/10.1016/j.forsciint.2006.08.020
Yoshimura, S., M. Yoshida, Y. Okii, T. Tokiyasu, T. Watabiki and A. Akane. 1995. “Detection of green algae (Chlorophyceae) for the diagnosis of drowning.” International Journal of Legal Medicine 108(1): 39–42. https://doi.org/10.1007/BF01845616
Young, J. M., L. S. Weyrich, J. Breen, L. M. Macdonald and A. Cooper. 2015. “Predicting the origin of soil evidence: High throughput eukaryote sequencing and MIR spectroscopy applied to a crime scene scenario. Forensic Science International 251: 22–31. https://doi.org/10.1016/j.forsciint.2015.03.008
Young, J. M., L. S. Weyrich and A. Cooper. 2016. “High–throughput sequencing of trace quantities of soil provides reproducible and discriminative fungal DNA profiles.” Journal of Forensic Sciences 61: 478–484. https://doi.org/10.1111/1556-4029.12996
Zimmerman, K. and J. R. Wallace. 2008. “Estimating a postmortem submersion interval using algal diversity on mammalian carcasses in brackish marshes.” Journal of Forensic Sciences 53: 935–941. https://doi.org/10.1111/j.1556-4029.2008.00748.x